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a) Log 2 ([elevated temperature/control] average total accumulation) of metabolic categories (n≥3) at six different seed developmental stages. b) Untargeted metabolomic data statistical analysis. c) Differentially accumulated metabolic features during seed development. d) Number of major metabolic categories (Flavonoids, Cinnamic acids and derivatives, and Glucosinolates) induced by elevated temperature at each seed developmental stage. e) <t>Transcriptomic</t> data statistical analysis. f) Differentially expressed genes during seed development. g) Percentages of genes coding for enzymes putatively involved in specialized metabolite modifications (Acyltransferases, Glycosyltransferases, Hydroxylases and Methyltransferases) induced, and repressed, at each seed developmental stage by elevated temperature.
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a) Log 2 ([elevated temperature/control] average total accumulation) of metabolic categories (n≥3) at six different seed developmental stages. b) Untargeted metabolomic data statistical analysis. c) Differentially accumulated metabolic features during seed development. d) Number of major metabolic categories (Flavonoids, Cinnamic acids and derivatives, and Glucosinolates) induced by elevated temperature at each seed developmental stage. e) <t>Transcriptomic</t> data statistical analysis. f) Differentially expressed genes during seed development. g) Percentages of genes coding for enzymes putatively involved in specialized metabolite modifications (Acyltransferases, Glycosyltransferases, Hydroxylases and Methyltransferases) induced, and repressed, at each seed developmental stage by elevated temperature.
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a) Log 2 ([elevated temperature/control] average total accumulation) of metabolic categories (n≥3) at six different seed developmental stages. b) Untargeted metabolomic data statistical analysis. c) Differentially accumulated metabolic features during seed development. d) Number of major metabolic categories (Flavonoids, Cinnamic acids and derivatives, and Glucosinolates) induced by elevated temperature at each seed developmental stage. e) <t>Transcriptomic</t> data statistical analysis. f) Differentially expressed genes during seed development. g) Percentages of genes coding for enzymes putatively involved in specialized metabolite modifications (Acyltransferases, Glycosyltransferases, Hydroxylases and Methyltransferases) induced, and repressed, at each seed developmental stage by elevated temperature.
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a) Log 2 ([elevated temperature/control] average total accumulation) of metabolic categories (n≥3) at six different seed developmental stages. b) Untargeted metabolomic data statistical analysis. c) Differentially accumulated metabolic features during seed development. d) Number of major metabolic categories (Flavonoids, Cinnamic acids and derivatives, and Glucosinolates) induced by elevated temperature at each seed developmental stage. e) <t>Transcriptomic</t> data statistical analysis. f) Differentially expressed genes during seed development. g) Percentages of genes coding for enzymes putatively involved in specialized metabolite modifications (Acyltransferases, Glycosyltransferases, Hydroxylases and Methyltransferases) induced, and repressed, at each seed developmental stage by elevated temperature.
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a) Log 2 ([elevated temperature/control] average total accumulation) of metabolic categories (n≥3) at six different seed developmental stages. b) Untargeted metabolomic data statistical analysis. c) Differentially accumulated metabolic features during seed development. d) Number of major metabolic categories (Flavonoids, Cinnamic acids and derivatives, and Glucosinolates) induced by elevated temperature at each seed developmental stage. e) Transcriptomic data statistical analysis. f) Differentially expressed genes during seed development. g) Percentages of genes coding for enzymes putatively involved in specialized metabolite modifications (Acyltransferases, Glycosyltransferases, Hydroxylases and Methyltransferases) induced, and repressed, at each seed developmental stage by elevated temperature.

Journal: bioRxiv

Article Title: Elevated temperature drives the biosynthesis of novel acylated glucosinolates in Arabidopsis thaliana seeds

doi: 10.64898/2026.06.03.729804

Figure Lengend Snippet: a) Log 2 ([elevated temperature/control] average total accumulation) of metabolic categories (n≥3) at six different seed developmental stages. b) Untargeted metabolomic data statistical analysis. c) Differentially accumulated metabolic features during seed development. d) Number of major metabolic categories (Flavonoids, Cinnamic acids and derivatives, and Glucosinolates) induced by elevated temperature at each seed developmental stage. e) Transcriptomic data statistical analysis. f) Differentially expressed genes during seed development. g) Percentages of genes coding for enzymes putatively involved in specialized metabolite modifications (Acyltransferases, Glycosyltransferases, Hydroxylases and Methyltransferases) induced, and repressed, at each seed developmental stage by elevated temperature.

Article Snippet: Untargeted metabolomic raw data (.mzXML) for both negative and positive ESI modes, and metadata have been deposited at the MassiVE data repository portal with the following identifiers: The transcriptomic RNA-Seq raw data (FASTQ) have been deposited at the National Center for Biotechnology Information (NCBI) Transcriptome Shotgun Assembly Sequence Database (TSA) with BioProject identification PRJNA1344327.

Techniques: Control, Metabolomic